Functional analysis of an epitope in the S2 subunit of the murine coronavirus spike protein: involvement in fusion activity.
Identifieur interne : 006473 ( Main/Exploration ); précédent : 006472; suivant : 006474Functional analysis of an epitope in the S2 subunit of the murine coronavirus spike protein: involvement in fusion activity.
Auteurs : Fumihiro Taguchi ; Yohko K. ShimazakiSource :
- The Journal of general virology [ 0022-1317 ] ; 2000.
Descripteurs français
- KwdFr :
- Animaux, Anticorps monoclonaux (immunologie), Anticorps monoclonaux (pharmacologie), Antigènes viraux (), Antigènes viraux (génétique), Antigènes viraux (immunologie), Antigènes viraux (métabolisme), Données de séquences moléculaires, Fusion cellulaire, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Glycoprotéines membranaires (métabolisme), Lignée cellulaire, Mutation (génétique), Protéines de fusion virale (), Protéines de fusion virale (génétique), Protéines de fusion virale (immunologie), Protéines de fusion virale (métabolisme), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Protéines de l'enveloppe virale (métabolisme), Récepteurs viraux (antagonistes et inhibiteurs), Récepteurs viraux (métabolisme), Séquence d'acides aminés, Tests aux précipitines, Tests de neutralisation, Virus de l'hépatite murine (), Virus de l'hépatite murine (génétique), Virus de l'hépatite murine (immunologie), Virus de l'hépatite murine (métabolisme), Épitopes (), Épitopes (génétique), Épitopes (immunologie), Épitopes (métabolisme).
- MESH :
- antagonistes et inhibiteurs : Récepteurs viraux.
- génétique : Antigènes viraux, Glycoprotéines membranaires, Mutation, Protéines de fusion virale, Protéines de l'enveloppe virale, Virus de l'hépatite murine, Épitopes.
- immunologie : Anticorps monoclonaux, Antigènes viraux, Glycoprotéines membranaires, Protéines de fusion virale, Protéines de l'enveloppe virale, Virus de l'hépatite murine, Épitopes.
- métabolisme : Antigènes viraux, Glycoprotéines membranaires, Protéines de fusion virale, Protéines de l'enveloppe virale, Récepteurs viraux, Virus de l'hépatite murine, Épitopes.
- pharmacologie : Anticorps monoclonaux.
- Animaux, Antigènes viraux, Données de séquences moléculaires, Fusion cellulaire, Glycoprotéine de spicule des coronavirus, Lignée cellulaire, Protéines de fusion virale, Séquence d'acides aminés, Tests aux précipitines, Tests de neutralisation, Virus de l'hépatite murine, Épitopes.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Monoclonal (immunology), Antibodies, Monoclonal (pharmacology), Antigens, Viral (chemistry), Antigens, Viral (genetics), Antigens, Viral (immunology), Antigens, Viral (metabolism), Cell Fusion, Cell Line, Epitopes (chemistry), Epitopes (genetics), Epitopes (immunology), Epitopes (metabolism), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Membrane Glycoproteins (metabolism), Molecular Sequence Data, Murine hepatitis virus (drug effects), Murine hepatitis virus (genetics), Murine hepatitis virus (immunology), Murine hepatitis virus (metabolism), Mutation (genetics), Neutralization Tests, Precipitin Tests, Receptors, Virus (antagonists & inhibitors), Receptors, Virus (metabolism), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Envelope Proteins (metabolism), Viral Fusion Proteins (chemistry), Viral Fusion Proteins (genetics), Viral Fusion Proteins (immunology), Viral Fusion Proteins (metabolism).
- MESH :
- chemical , antagonists & inhibitors : Receptors, Virus.
- chemical , chemistry : Antigens, Viral, Epitopes, Viral Fusion Proteins.
- chemical , genetics : Antigens, Viral, Epitopes, Membrane Glycoproteins, Viral Envelope Proteins, Viral Fusion Proteins.
- chemical , immunology : Antibodies, Monoclonal, Antigens, Viral, Epitopes, Membrane Glycoproteins, Viral Envelope Proteins, Viral Fusion Proteins.
- chemical , metabolism : Antigens, Viral, Epitopes, Membrane Glycoproteins, Receptors, Virus, Viral Envelope Proteins, Viral Fusion Proteins.
- chemical , pharmacology : Antibodies, Monoclonal.
- drug effects : Murine hepatitis virus.
- genetics : Murine hepatitis virus, Mutation.
- immunology : Murine hepatitis virus.
- metabolism : Murine hepatitis virus.
- Amino Acid Sequence, Animals, Cell Fusion, Cell Line, Molecular Sequence Data, Neutralization Tests, Precipitin Tests, Spike Glycoprotein, Coronavirus.
Abstract
The monoclonal antibody (MAb) 5B19.2, which has virus-neutralizing and fusion inhibition activities, binds to an epitope (S2A) consisting of nine hydrophobic amino acids in the S2 subunit of the mouse hepatitis virus (MHV) spike (S) protein. This suggests that the S2A epitope may be involved in binding the virus to the MHV receptor and/or in virus-cell fusion. Co-immunoprecipitation analyses demonstrated that while the binding of virus to the receptor was blocked by anti-S1 MAbs, it was not blocked by the S2A antiserum, indicating that S2A was not involved in receptor-binding. The S proteins prepared in this study with mutations in the S2A epitope were either fusogenic or non-fusogenic and their fusogenicity did not correlate with the hydrophobic feature of the S2A epitope. All of these wt and mutated S proteins were similarly transported onto the cell membrane independent of their fusogenicity capability. These results suggest that S2A may mediate the fusion activity of the MHV S protein during virus entry into cells.
DOI: 10.1099/0022-1317-81-12-2867
PubMed: 11086117
Affiliations:
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Le document en format XML
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(metabolism)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (immunology)</term><term>Membrane Glycoproteins (metabolism)</term><term>Molecular Sequence Data</term><term>Murine hepatitis virus (drug effects)</term><term>Murine hepatitis virus (genetics)</term><term>Murine hepatitis virus (immunology)</term><term>Murine hepatitis virus (metabolism)</term><term>Mutation (genetics)</term><term>Neutralization Tests</term><term>Precipitin Tests</term><term>Receptors, Virus (antagonists & inhibitors)</term><term>Receptors, Virus (metabolism)</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term><term>Viral Envelope Proteins (metabolism)</term><term>Viral Fusion Proteins (chemistry)</term><term>Viral Fusion Proteins (genetics)</term><term>Viral Fusion Proteins (immunology)</term><term>Viral Fusion Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps monoclonaux (immunologie)</term><term>Anticorps monoclonaux (pharmacologie)</term><term>Antigènes viraux ()</term><term>Antigènes viraux (génétique)</term><term>Antigènes viraux (immunologie)</term><term>Antigènes viraux (métabolisme)</term><term>Données de séquences moléculaires</term><term>Fusion cellulaire</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Glycoprotéines membranaires (métabolisme)</term><term>Lignée cellulaire</term><term>Mutation (génétique)</term><term>Protéines de fusion virale ()</term><term>Protéines de fusion virale (génétique)</term><term>Protéines de fusion virale (immunologie)</term><term>Protéines de fusion virale (métabolisme)</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Récepteurs viraux (antagonistes et inhibiteurs)</term><term>Récepteurs viraux (métabolisme)</term><term>Séquence d'acides aminés</term><term>Tests aux précipitines</term><term>Tests de neutralisation</term><term>Virus de l'hépatite murine ()</term><term>Virus de l'hépatite murine (génétique)</term><term>Virus de l'hépatite murine (immunologie)</term><term>Virus de l'hépatite murine (métabolisme)</term><term>Épitopes ()</term><term>Épitopes (génétique)</term><term>Épitopes (immunologie)</term><term>Épitopes (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Receptors, Virus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antigens, Viral</term><term>Epitopes</term><term>Viral Fusion Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, 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xml:lang="en"><term>Murine hepatitis virus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Murine hepatitis virus</term><term>Mutation</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Antigènes viraux</term><term>Glycoprotéines membranaires</term><term>Mutation</term><term>Protéines de fusion virale</term><term>Protéines de l'enveloppe virale</term><term>Virus de l'hépatite murine</term><term>Épitopes</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps monoclonaux</term><term>Antigènes viraux</term><term>Glycoprotéines membranaires</term><term>Protéines de fusion virale</term><term>Protéines de l'enveloppe virale</term><term>Virus de l'hépatite murine</term><term>Épitopes</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Murine hepatitis virus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Murine hepatitis virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antigènes viraux</term><term>Glycoprotéines membranaires</term><term>Protéines de fusion virale</term><term>Protéines de l'enveloppe virale</term><term>Récepteurs viraux</term><term>Virus de l'hépatite murine</term><term>Épitopes</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Anticorps monoclonaux</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Cell Fusion</term><term>Cell Line</term><term>Molecular Sequence Data</term><term>Neutralization Tests</term><term>Precipitin Tests</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Antigènes viraux</term><term>Données de séquences moléculaires</term><term>Fusion cellulaire</term><term>Glycoprotéine de spicule des coronavirus</term><term>Lignée cellulaire</term><term>Protéines de fusion virale</term><term>Séquence d'acides aminés</term><term>Tests aux précipitines</term><term>Tests de neutralisation</term><term>Virus de l'hépatite murine</term><term>Épitopes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The monoclonal antibody (MAb) 5B19.2, which has virus-neutralizing and fusion inhibition activities, binds to an epitope (S2A) consisting of nine hydrophobic amino acids in the S2 subunit of the mouse hepatitis virus (MHV) spike (S) protein. This suggests that the S2A epitope may be involved in binding the virus to the MHV receptor and/or in virus-cell fusion. Co-immunoprecipitation analyses demonstrated that while the binding of virus to the receptor was blocked by anti-S1 MAbs, it was not blocked by the S2A antiserum, indicating that S2A was not involved in receptor-binding. The S proteins prepared in this study with mutations in the S2A epitope were either fusogenic or non-fusogenic and their fusogenicity did not correlate with the hydrophobic feature of the S2A epitope. All of these wt and mutated S proteins were similarly transported onto the cell membrane independent of their fusogenicity capability. These results suggest that S2A may mediate the fusion activity of the MHV S protein during virus entry into cells.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Shimazaki, Yohko K" sort="Shimazaki, Yohko K" uniqKey="Shimazaki Y" first="Yohko K" last="Shimazaki">Yohko K. Shimazaki</name><name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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